Vitamin D, 25-Hydroxy (Calcifediol) Test
Price: $35
Why Test Vitamin D?
Vitamin D deficiency is one of the most common and under-recognized causes of fatigue, low mood, inflammation, bone pain, immune weakness, and poor recovery from illness. Low levels also contribute to impaired methylation, serotonin imbalance, and chronic inflammatory conditions. Because symptoms are vague, the only reliable way to know your vitamin D status is through a blood test.
The 25-Hydroxyvitamin D (Calcifediol) test is the gold-standard screening method used by clinicians to determine total vitamin D stores in the body. Calcifediol remains stable in circulation and accurately reflects both dietary intake and sunlight exposure.
What This Test Measures
This LabCorp test evaluates:
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Total 25-Hydroxyvitamin D (Calcifediol) — the most accurate marker of vitamin D status.
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Reflects vitamin D from sun exposure, food, and supplements.
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Helps determine whether deficiency, insufficiency, or optimal levels are present.
This test does not measure calcitriol (1,25-D), which fluctuates based on kidney regulation and is not a reliable indicator of vitamin D deficiency.
Why 25-Hydroxy (Calcifediol) Is the Correct Test
Many patients mistakenly ask for calcitriol. Physicians trained in nutritional biochemistry—including Walsh Protocol clinicians—prefer calcifediol because:
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Higher stability and longer half-life (approx. 2 weeks).
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Accurate reflection of vitamin D stores, not momentary hormonal shifts.
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Clinically reliable for supplement decisions and tracking improvements over time.
This is the exact test used in research linking vitamin D status to inflammation, immune health, serotonin signaling, and chronic disease risk.
Who Should Consider This Test?
Testing is recommended if you experience:
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Persistent fatigue or low energy
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Muscle aches, bone pain, or weakness
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Frequent illness or slow recovery
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Low mood, anxiety, or seasonal depression
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Autoimmune conditions
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Poor methylation, high homocysteine, or high inflammation markers
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Limited sun exposure or darker skin tone
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Vegan or low-fat diet
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Obesity or chronic illness (vitamin D is absorbed into fat stores)
What You Receive
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A LabCorp order (no doctor’s visit required)
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Testing at any LabCorp location nationwide
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Results typically within 2–3 business days
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Optional review and interpretation through Second Opinion Physician services
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Guidance on optimal ranges, not just “normal” lab values
How to Use This Test
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Add the test to your cart and complete checkout.
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You will receive a LabCorp requisition form by email.
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Visit any LabCorp site for a quick blood draw.
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Results will be delivered securely through your patient portal.
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You may schedule a follow-up consult for interpretation and a personalized plan.
Optimal Levels
While labs often report wide “normal” ranges, research and clinical practice commonly support:
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50–90 ng/mL as an optimal functional range
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Levels below 30 ng/mL indicate deficiency
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Levels below 20 ng/mL significantly increase risk of infection, mood issues, and bone loss









